“With the majority of biotherapeutic drugs manufactured in CHO cell lines, expressing small-scale therapeutic antibody candidates in CHO for screening has become a vital first step to ensure successful downstream scale-up. We now provide a comprehensive portfolio of antibody production to further serve the biopharma market by offering both HEK293 with reduced turnaround times enabled by our Express Genes as well as the newly launched CHO cell line for Twist Express Antibodies,” said Emily M. Leproust, Ph.D., CEO and co-founder of Twist Bioscience. “By taking on the process of antibody development from sequence to protein, we meet the needs of therapeutic developers who currently make their own antibodies, a costly bottleneck in the discovery pipeline, providing them with a cost-effective, customizable, and scalable offering to support their research.”

Using its high-throughput antibody production workflow, Twist can generate large quantities of small-scale distinct antibodies for candidate and drug discovery screening. Antibodies are created from user-defined sequences using the customer’s expression system of choice, either CHO or HEK293. Twist Express Antibodies are sequence-perfect and high quality, delivered in as few as 10 business days.

Twist Express Antibodies, CHO

Twist Express Antibodies, CHO, are developed using a high-throughput antibody production workflow that yields IgG antibodies in quantities ranging from 15 μg up to 3,000 μg. The antibodies are made using sequence-verified perfect clonal genes for high quality. Twist also provides yield data on each antibody sequence to be used as a screening tool for candidate selection as well as control yield data to provide peace of mind and confidence of successful transfection and protein production. Turnaround time for Twist Express Antibodies, CHO, starts at 13 business days.

Twist Express Antibodies

Twist Express Antibodies provide a comprehensive and efficient solution to antibody production and purification. The process begins with a customer entering antibody sequences into Twist’s eCommerce ordering platform, where bioinformatic screening and gene scoring is used to filter out potentially non-viable sequences. From there, synthetic DNA is generated, which is either cloned into one of Twist’s IgG scaffold vectors, or a vector of the customer’s choice. Twist’s silicon-based DNA synthesis platform enables the simultaneous production of up to thousands of antibody genes, which enables flexible production of tens to thousands of distinct antibodies. Twist sequences the antibody genes to ensure that the precise order has been cloned correctly, every time. These antibodies are then produced in CHO or HEK293 cells, resin purified, and have a turnaround time starting at 10 business days for HEK293 and 13 business days for CHO