Ecnoglutide is a novel, fatty acid-conjugated long-acting GLP-1 analog consisting of only natural amino acids, which simplifies the manufacturing process compared to some other GLP-1 analogs. In vitro, ecnoglutide showed potent activity that is biased towards cAMP induction over GLP-1 receptor internalization. In rodent models, ecnoglutide showed significant improvements in glycemic control and body weight reduction compared to semaglutide. In a Phase 1 clinical study, ecnoglutide was found to be generally safe and well tolerated with pharmacokinetic properties supporting once-weekly subcutaneous injections.